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KMID : 0545120140240060869
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Journal of Microbiology and Biotechnology
2014 Volume.24 No. 6 p.869 ~ p.878
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Identification of Lactoferrin as a Human Dedifferentiation Factor Through the Studies of Reptile Tissue Regeneration Mechanisms
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Bae Kil-Soo
Kim Sun-Young
Park Soon-Yong
Jeong Ae-Jin
Lee Hyun-Hee
Lee Jung-Woon
Cho Yee-Sook
Leem Sun-Hee
Kang Tae-Hong
Bae Kwang-Hee
Kim Jae-Ho
Jung Yong-Woo
Jun Woo-Jin
Yoon Suk-Ran
Lee Sang-Chul
Chung Jin-Woong
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Abstract
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In this study, we performed two-dimensional electrophoresis with protein extracts from lizard tails, and analyzed the protein expression profiles during the tissue regeneration to identify the dedifferentiation factor. As a result, we identified 18 protein spots among total of 292 spots, of which proteins were specifically expressed during blastema formation. We selected lactoferrin as a candidate because it is the mammalian homolog of leech-derived tryptase inhibitor, which showed the highest frequency among the 18 proteins. Lactoferrin was specifically expressed in various stem cell lines, and enhanced the efficiency of iPSC generation upto approximately 7-fold relative to the control. Furthermore, lactoferrin increased the efficiency by 2-fold without enforced expression of Klf4. These results suggest that lactoferrin may induce dedifferentiation, at least partly by increasing the expression of Klf4.
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KEYWORD
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dedifferentiation, iPSCs, lactoferrin, stem cell, tissue regeneration
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